During the car trip to her new home, the introverted flower lover Hanasaki Tsubomi has a strange and mysterious dream, where the legendary warrior known as Cure Moonlight is defeated at the base of the "Great Tree of Hearts". Because of this, all the beautiful plants are destroyed, and two fairies from the Tree of Hearts take off.
It soon turns out to be real, and the fairies appear next to her. They want her to become the legendary warrior Pretty Cure and collect Heart Seeds in order to prevent the Great Tree of Hearts, the source of all human hearts, from dying. But Tsubomi, unsure of herself, thinks she is unable to do so and refuses. However, an enemy attacks her classmate Kurumi Erika and takes away her Heart Flower. To save Erika's heart, Tsubomi summons the courage she needs and transforms into the Pretty Cure warrior, Cure Blossom.
- Heartcatch Pretty Cure! episodes
- Heartcatch Pretty Cure!: Hana no Miyako de Fashion Show... Desu ka!?
- Hanasaki Tsubomi (花咲つぼみ Hanasaki Tsubomi?): A very shy and introverted girl who loves flowers and dreams of being a botanist in order to turn all deserts of the world into flower gardens. She lives in a flower shop called "Flower Shop Hanasaki" with her parents and grandmother. She loves her grandmother and has picked up her old-fashioned speech pattern. Her catchphrase during battle is "I've had ENOUGH!". Her theme color is pink, and her alter ego is Cure Blossom (キュアブロッサム Kyua Burossamu?). Her powers are related to all types of flowers, specifically cherry blossoms.
- Kurumi Erika (来海えりか Kurumi Erika?): She loves fashion and dreams of being a fashion designer. Her home is a fashion shop called "Fairy Drop", and she lives with both her parents, plus her older sister who is a fashion model. Her theme color is blue, and her alter ego is Cure Marine (キュアマリン Kyua Marin?). Her powers are related to blue daisies and water.
- Myoudouin Itsuki (明堂院いつき Myōdōin Itsuki?): Student council president of Myoudou Academy's Junior High School, Itsuki is also the granddaughter of the president of the school. With a gentle and handsome appearance, she is always crowded by subordinates and fans wherever she goes. Although a girl, Itsuki is often mistaken as a boy because of family matters. Wanting to protect her older brother, Itsuki is accepted by Potpourri as a Pretty Cure, and she turns into Cure Sunshine (キュアサンシャイン Kyua Sanshain?). Her powers are related to sunlight and sunflowers.
- Tsukikage Yuri (月影ゆり Tsukikage Yuri?): Privately, Yuri is Erika's older sister Momoka's best friend, helping her at school whenever Momoka cannot make it because of work. However, before the series started, she was the last of the previous generation of Pretty Cure, Cure Moonlight. With the help of Cure Blossom, Cure Marine, Cure Sunshine, Cologne, and the Heart Tree she transforms back into Cure Moonlight (キュアムーンライト Kyua Mūnraito?). Her powers are related to moonlight and silver roses.
- Chypre (シプレ Shipure?): A fairy from the Tree of Hearts, and Tsubomi's companion. She shows a great amount of affection for Tsubomi and is the one to hug Tsubomi when she is feeling low and incapable.
- Coffret (コフレ Kofure?): Another fairy from the Great Tree of Hearts, and Erika's companion. More cheeky towards Erika than any other mascot is towards their partner. He feared Erika did not appreciate him at one point. This caused him to steal her flower tact only for the Snackys to get hold of it. When the Snackys hurt him in battle Erika revealed to him just how important he is to her.
- Master Coupe (コッペ様 Koppe-sama?): A superior fairy who is greatly admired by other fairies such as Chypre and Coffret, who lives in the botanical garden that Tsubomi's grandmother cares for. He does not talk but is always watching over Tsubomi and Erika. In the past, he was the companion of Kaoruko when she was Cure Flower. He has the ability to transform into Mysterious Guy. Coupe was also the one to rescue Yuri after she lost her powers in a fight against Dark Cure.
- Potpourri (ポプリ Popuri?): A baby fairy from the Tree of Hearts, Potpourri possesses incredible protecting powers, and is born after Pretty Cure have managed to collect enough Heart Seeds. She is the partner of Cure Sunshine, but not before having to look for her, candidate after candidate. Potpourri also worries about the heart tree more than the other mascots, this is shown on a few occasions such as when Dark Cure made the flowers of the tree fall off.HPC21
- Cologne (コロン Koron?): Cologne was Yuri's partner, but he died while trying to protect her, Chypre, Coffret and the Tree of Hearts. He tried to encourage Yuri to have companions, something she did not feel she needed. Despite this, he stood by his partner, which led to his sacrifice to save Yuri.HPC33
- Desertrian (デザトリアン Dezatorian?): Desertrians are the monsters used by the Desert Apostles, created by fusing an object with the wilting Heart Flower of a person.
- Kumojaki (クモジャキー Kumojakī?): The apparent leader of the three managers of the Desert Apostles. He trains a lot and sometimes shown wielding a sword.
- Sasorina (サソリーナ Sasorīna?): Only female of the three managers of the Desert Apostles. She is a direct person and battles Pretty Cure with the fullest of her feelings. She can attack with her hair, like a scorpion tail.
- Cobraja (コブラージャ Koburāja?): The third member of the three managers of the Desert Apostles. He is a strong narcissist who thinks he is the most beautiful man in the universe. He always tries to look as beautiful as possible, even in battle.
- Snackey (スナッキー Sunakkī?): The underlings of the Desert Apostles, always hard-working. Cobraja seems to make the most use of them, having them assist him with things such as lighting and photography for impromptu photo shoots during his missions. In episode 39, the leader of the Snackeys, Boss Snackey, appears.
- Desert Devil: The Desert Devil is a powerhouse in Dune's army. He sends the Desert Devil to a thriving planet full of life, allowing it to turn the planet into an endless lifeless desert.
- Dark Cure (ダークプリキュア Dāku Purikyua?): A mysterious woman who has powers very much like those of Pretty Cure, including her own Tact. It is unknown if she is a real Pretty Cure or if she was merely created by Professor Sabaku to resemble and act like one. She has complete heterochromia, her left eye is light blue while her right eye, which she usually keeps closed, is yellow.
- Professor Sabaku (サバーク博士 Sabāku-hakase?): The acting main villain in the series. He is very mysterious, constantly keeping his face hidden beneath a mask. He usually has Dark Cure by his side.
- Dune (デューン Dyūn?): The actual leader of the Desert Apostles, who has been in deep sleep after he was defeated by Cure Flower.HPC20
Myoudou Academy Fashion Club
- Sawai Naomi (沢井なおみ Sawai Naomi?)
- Sakuma Toshiko (佐久間としこ Sakuma Toshiko?)
- Kuroda Rumiko (黒田るみこ Kuroda Rumiko?)
- Shiku Nanami (志久ななみ Shiku Nanami?): Acting mother for her household, in the absence of her late mother. She joins the fashion club after Erika and Tsubomi (with the help of Nanami's little sister, Shiku Rumi) convince her that she needs to have her own life, too.
- Hanasaki Kaoruko (花咲薫子 Hanasaki Kaoruko?): Tsubomi's grandmother, who Tsubomi often used to visit and stay with in the past. She used to be Pretty Cure in her youth, her Cure alias being Cure Flower (キュアフラワー Kyua Furawā?).
- Kurumi Momoka (来海ももか Kurumi Momoka?): Erika's 17-year-old sister. She is a charisma model for a fashion magazine and the source of Erika's complex, as she thinks Momoka is much more beautiful and mature than her. Her best friend/classmate is Yuri.
- Myoudouin Satsuki (明堂院さつき Myōdōin Satsuki?): Itsuki's older brother. He is sickly and hence Itsuki has to carry on their family tradition.
- Myoudouin Gentarou (明堂院厳太郎 Myōdōin Gentarō?): Itsuki's grandfather, he's the principal of Myoudou Academy and the leader of the town's local dojo.
- Myoudouin Tsubaki (明堂院つばき Myōdōin Tsubaki?): Itsuki's mother, she's the actual daughter of the principal Myoudouin Gentarou.
- Tsurusaki (鶴崎先生 Tsurusaki-sensei?): Tsubomi and Erika's homeroom teacher in Myōdō Academy, strict but reliable. She is afraid of ghosts, which she thinks is holding her back from her teaching.
Light Music Club
- Ikeda Aya: Aya is a minor character in episode 36. She sings HEART GOES ON with her partner in the light music club, Kudou Mayu.
- Kudou Mayu: Mayu is a minor character in episode 1 and episode 36. She sings HEART GOES ON with her partner in the light music club, Ikeda Aya.
- Heart Perfumes (ココロパフューム Kokoro Pafyūmu?) - Perfume bottles used to transform. Cure Sunshine has a Heart Perfume which is actually called the Shiny Perfume.
- Flower Tacts (フラワータクト Furawā Takuto?) - Wand like items used as weapons. The different types of Flower Tacts are named after their users; the Blossom Tact for Cure Blossom, the Marine Tact for Cure Marine, the Moon Tact for Cure Moonlight and the Dark Tact for Dark Cure. Cure Sunshine has an attack device called the Shiny Tambourine, but it is unknown if this is a Flower Tact.
- Heart Pot (ココロポット Kokoro Potto?) - The item that the Heart Seeds are stored in. Later in episode 33, the lid of the Heart Pot is used by Yuri to transform into Cure Moonlight.
- Heart Seeds (こころの種 Kokoro no Tane?, Seed of Heart) - They are born from cleansing people's Heart Flowers. They are stored in the Heart Pot. Some can be used for power-ups and calming the mind.
- Heartcatch Mirage (ハートキャッチミラージュ Hātokyacchi Mirāju?) - Is a jewelry box-like item that was used by all predecessors of Pretty Cures. It is extremely powerful and gives the Pretty Cures a boost of power, the ability to transport directly to the Tree of Hearts, show one's Heart Flowers and part of the Cure's Super Silhouette transformation. It resides in the Pretty Cure Palace, in which the Pretty Cure who ask for it will have to face the Pretty Cure before them.
- Shiny Tambourine (シャイニータンバリン Shainī Tanbarin?) - Is Cure Sunshine's main weapon. Different from the Flower Tact, its design is similar to a tambourine shaped like a sunflower. Allows her to use Gold Forte Burst and Shining Fortissimo along with Cure Blossom and Cure Marine.
- Myoudou Academy's Junior High School (私立明堂学園 Shiritsu Myōdō Gakuen?) is the school that Tsubomi, Erika, Itsuki, and Yuri attend.
- Kibougahana (希望ヶ花 Kibōgahana?) is the town that Heartcatch Pretty Cure! is set in.
- Flower Shop Hanasaki (フラワーショップ花咲 Furawā Shoppu Hanasaki?) is Tsubomi, her parents and grandmother's flower shop and house.
- Fairy Drop (フェアリードロップ Fearī Doroppu?) is a two-story fashion store that sells accessories on the first floor and clothes on the second floor. The shop is run by Erika's parents and is connected to the Kurumi household, with Erika's room on the second floor. Clothing bought from Fairy Drop usually has the store's ribbon logo on it. It's located right next door to Flower Shop Hanasaki.
- Myoudouin Kobudo (明堂院流古武道 Myōdōin Kobudo?) is the martial arts studio home of the Myoudouin family, including daughter Itsuki.
- Desert Apostles (砂漠の使徒 Sabaku no Shito?) is the Desert Apostles base that consists of Kumojacky, Cobraja, Sasorina, Sabaku, Dark Pretty Cure, Baron Salamander (movie exclusive) and the leader Dune.
- The song HEART GOES ON by Ikeda Aya and Kudou Mayu is one of the main insert themes for Heartcatch Pretty Cure!.
- Heartcatch Pretty Cure! is the first season to have sub-attacks (not including basic attacks, such as double punches and kicks like in Fresh Pretty Cure!).
- In Heartcatch Pretty Cure! all the main Cures sing their respective character songs as insert songs:
- Heartcatch Pretty Cure! is the first Pretty Cure series that received an afureko (after recording). Mizuki Nana (voice actress of Hanasaki Tsubomi) was a guest in Shin Domoto Kyodai.
- Heartcatch Pretty Cure! had its opening theme performed in The 61st NHK Kouhaku Uta Gassen (NHK Red White Music Festival). The performers are Maeda Atsuko, Ooshima Yuuko, Itano Tomomi (ex-AKB48) and Mizuki Nana (voice actress of Hanasaki Tsubomi)
- A Heartcatch Pretty Cure! the poster appears in the final episode of Imagin Anime, a spin-off of the Tokusatsu series Kamen Rider Den-O. Momotaros complains about the poster's size and him being left out of the poster.
- Main article: Heartcatch Pretty Cure! Merchandise